The inactivation efficiency with traditionally used techniques such as chlorination will be tested in parallel to evaluate advantages of the new technique. One of the drawbacks of traditionally used virus inactivation methods like chlorination is the release of disinfection by-products. Here we will evaluate the potential effect of the water treated with both cavitation and plasma on plant’s growth and development, toxicity to human cells and compare it with the effects of a chemical treatment i.e., chlorination. In collaboration with the project partner at the wastewater treatment plant Domžale, we will also asses the suitability for upscaling the technology to a water treatment plant level, so commercialization of the technology is foreseen after termination of this project. This could be achieved with the involvement of the Ministry of agriculture, forestry and food, which is included as a financer. The dissemination of the project results will raise public awareness and alert the responsible stakeholders to the waterborne microbe problematics and solutions offered by the project. This is especially important in the field of reusable water resources management, which is more and more important due to climate change and the lack of high quality water resources.